If more substrate to already occurring enzymatic reaction is added more enzyme activity is seen because_____________?
Correct answer: B. There is probably more enzayme available than there is substrate.
- A. There is probably more substrate present than there is enzyme.
- B. There is probably more enzayme available than there is substrate.
- C. There is probably more product present than there is either substrate or enzyme.
- D. The enzyme substrate complex is probably failing to form during the reaction
Explanation
If adding substrate increases the rate, the original reaction had substrate below the level needed to occupy all available active sites, so some enzyme was still available. The likely mistake is choosing a, but that would describe a situation where substrate was already in excess and adding more had little or no effect.
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About Factors Affecting Enzyme Action
Enzyme activity changes with temperature, pH, substrate concentration and enzyme concentration because these factors affect molecular collisions and the active site. Questions also examine optimum conditions, denaturation and inhibition, while distinguishing a fall in activity caused by unsuitable conditions from permanent enzyme damage.
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