Which bacterial structure is primarily responsible for retaining crystal violet during Gram staining in Gram-positive bacteria?

Correct answer: C. A thick peptidoglycan layer

  • A. A thin peptidoglycan layer
  • B. An outer lipopolysaccharide membrane
  • C. A thick peptidoglycan layer
  • D. A capsule rich in polysaccharides

Explanation

Gram-positive bacteria have a thick peptidoglycan layer that traps the crystal violet iodine complex during decolorization. Gram-negative bacteria instead possess a thin peptidoglycan layer and an outer membrane containing lipopolysaccharide.

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