What is the correct sequence of steps in cell fractionation?
Correct answer: A. Homogenisation, centrifugation
- A. Homogenisation, centrifugation
- B. Centrifugation, Homogenisation
- C. Both A and B
- D. None of the above
Explanation
Cell fractionation is a process designed to separate and isolate cellular components, such as organelles. The first step is homogenization, where cells are broken open to release their contents. This is followed by centrifugation, where the homogenate is spun at various speeds to separate the components based on their size and density. Option A correctly describes this sequence. Options B, C, and D are incorrect because they do not reflect the necessary order of operations in cell fractionation.
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Animal, plant, prokaryotic and eukaryotic cells are compared through their membranes, cell walls, nuclei and cytoplasmic organelles. The work includes the structures and functions of mitochondria, chloroplasts, ribosomes, endoplasmic reticulum, Golgi apparatus and lysosomes, as well as chromosome organization and the distinction between chromatin and chromosomes.
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