What is needed to transcribe DNA?
Correct answer: D. RNA polymerase
- A. DNA ligase
- B. DNA polymerase
- C. Ribosomes
- D. RNA polymerase
Explanation
Transcription has three stages: initiation, elongation, and termination. During initiation, RNA polymerase binds to a sequence of DNA called the promoter, found near the beginning of a gene. Each gene (or group of co-transcribed genes, in bacteria) has its own promoter. Once bound, RNA polymerase separates the DNA strands, providing the single-stranded template needed for transcription. DNA polymerase is responsible for the process of DNA replication, during which a double-stranded DNA molecule is copied into two identical DNA molecules. DNA ligase is a DNA-joining enzyme used in genetic engineering. If two pieces of DNA have matching ends, ligase can link them to form a single, unbroken molecule of DNA. In translation, messenger RNA (mRNA) is decoded in a ribosome, outside the nucleus, to produce a specific amino acid chain, or polypeptide; this mRNA is created during transcription. The correct answer is, therefore, D.
This question appeared on the HEC Phase II 2017 paper, which you can sit online with every answer explained.
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About Ribonucleic Acid (RNA)
Ribonucleic acid is generally a single-stranded nucleic acid containing ribose and uracil instead of thymine. Coverage includes mRNA, tRNA and rRNA, complementary base pairing, transcription and translation, and the differences between RNA and DNA in structure, location and biological function.
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