What criterion is for DNA fragment movement on agarose gel during gel electrophoresis?
Correct answer: A. The smaller the fragment size, the farther it moves
- A. The smaller the fragment size, the farther it moves
- B. Positively charged fragments move to farther end
- C. Negatively charged fragments do not move
- D. The larger the fragment size, the farther it moves
Explanation
Electrophoresis is a technique used for the separation of substances of different ionic properties. Since the DNA fragments are negatively charged molecules, they can be separated by allowing them to move towards the anode. DNA fragments move towards the anode according to their molecule's size through the pores of agarose gel. Thus, the smaller fragments move farther away as compared to larger fragments.
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