A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using:
Correct answer: D. Ligase
- A. EcoRI
- B. Taq polymerase
- C. Polymerase II
- D. Ligase
Explanation
Ligase is a class of enzymes that catalyse the formation of covalent bonds using the energy released by the cleavage of ATP. Ligases are important in the synthesis and repair of many biological molecules, including DNA ligase and used in genetic engineering to insert foreign DNA into cloning vectors.
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Biotechnology in health care applies biological organisms, cells or enzymes to diagnose and treat disease. The subject includes recombinant DNA, genetically engineered insulin, vaccines, antibiotics, monoclonal antibodies, gene therapy and techniques such as polymerase chain reaction, while distinguishing therapeutic applications from agricultural and industrial biotechnology.
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