To obtain a human gene without its introns, genetic engineers make a DNA copy of the mature messenger RNA using
Correct answer: C. reverse transcriptase
- A. DNA ligase
- B. restriction endonuclease
- C. reverse transcriptase
- D. DNA helicase
Explanation
Reverse transcriptase, taken from retroviruses, builds a complementary DNA strand on an RNA template, and since the introns have already been spliced out of mature mRNA the resulting cDNA is a continuous coding sequence. This matters because a bacterium has no splicing machinery and could not process a gene containing introns. Ligase and restriction enzymes join and cut DNA but cannot copy RNA.
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Biotechnology applies genetic engineering, cell culture and recombinant DNA methods to medicine and public health. The material includes production of insulin, vaccines and antibiotics, diagnostic techniques, gene therapy, genetically modified organisms and the ethical concerns surrounding manipulation of human genes.
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