The tissue culture method occur in the following sequence:

Explanation

The correct sequence of the tissue culture method is: Media preparation -> Inoculation -> Sterilization -> Callus development -> Plantlets. Media preparation is the first step where nutrients are provided for growth. Inoculation involves introducing the inoculum into the prepared media. Sterilization is critical to ensuring a contaminant-free environment, which is necessary before callus development. Callus development involves the growth of undifferentiated cells, eventually leading to the formation of plantlets. Other options are incorrect because they misplace the crucial steps in the process.