The antibiotic resistance gene carried on a plasmid vector serves to

Correct answer: D. identify the bacteria that have successfully taken up the plasmid

  • A. cut the DNA at specific points
  • B. digest the bacterial cell wall
  • C. colour the bacterial colonies
  • D. identify the bacteria that have successfully taken up the plasmid

Explanation

Only bacteria carrying the plasmid survive on antibiotic containing medium, so the marker selects the transformed cells. The cutting is done by the restriction enzyme before the plasmid enters the cell.

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Biotechnology applies genetic engineering, cell culture and recombinant DNA methods to medicine and public health. The material includes production of insulin, vaccines and antibiotics, diagnostic techniques, gene therapy, genetically modified organisms and the ethical concerns surrounding manipulation of human genes.

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