Temperature-insensitive (thermostable) enzyme used in PCR is:
Correct answer: D. Taq polymerase
- A. DNA polymerase I
- B. DNA ligase
- C. DNA polymerase ITI
- D. Taq polymerase
Explanation
Explanation:Taq polymerase is very thermo stable as it is derived from bacteria Thermophilus aquaticus that lives in hot geysers. DNA polymerase l is an enzyme involved in DNA replication, repair, and recombination. It has a relatively low thermostability and is not commonly used in polymerase chain reaction (PCR).DNA Ligase is an enzyme that catalyzes the formation of phosphodiester bonds between adjacent DNA strands. It is not used as a thermostable enzyme in PCR.DNA polymerase lll is the primary enzyme involved in DNA replication in bacteria. It has a moderate level of thermostability but is not commonly used in PCR
This question appeared on the UHS MDCAT 2009 paper, which you can sit online with every answer explained.
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