Gene can be synthesized in laboratory from messenger RNA by using:
Correct answer: D. Reverse transcriptase
- A. Restriction enzymes
- B. cDNA (complementary DNA)
- C. Vector
- D. Reverse transcriptase
Explanation
[A] Restriction enzymes recognize a specific sequence of nucleotides in double stranded DNA and cut the DNA at a specific location [B] CDNA is incorrect as it is generated using reverse transcriptase.[C] Vector is the molecule that carries the recombinant DNA and this DNA is introduced into host for gene expression[D] Reverse transcriptase is an enzyme used to generate complementary DNA[cDNA] from an RNA template.
This question appeared on the UHS MDCAT 2010 paper, which you can sit online with every answer explained.
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