Moderate

Semi­-conservative replication of DNA was first demonstrated in:

Correct answer: A. Escherichia coli

  • A. Escherichia coli
  • B. Streptococcus pneumoniae
  • C. Salmonella typhimurium
  • D. Drosophila melanogaster

Explanation

The semi-conservative replication of DNA was first experimentally demonstrated by Matthew Meselson and Franklin Stahl in 1958. They used the bacterium Escherichia coli (E. coli) for their classic experiment, in which they used heavy nitrogen isotope (15N) and light nitrogen isotope (14N) to label and track the DNA strands across generations. The results of their density gradient centrifugation analysis confirmed the prediction of the Watson-Crick model that DNA replication is semi-conservative.

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