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Meselson and Stahl transferred few bacteria grown in N15 medium to N14 medium for replicating their DNA. What would be the result after two rounds of replication?

Correct answer: D. 50% hybrid duplex and 50% light duplex

  • A. 100% heavy duplex
  • B. 100% hybrid duplex
  • C. 50% hybrid duplex and 50% heavy duplex
  • D. 50% hybrid duplex and 50% light duplex

Explanation

After two rounds of replication, half of the duplexes will be hybrid i.e. contain one N15 strand and one N14 strand, while the other half will contain only N14 or light strands.

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