In an experiment on enzyme activity a buffer solution is used in order to
Correct answer: C. keep the pH constant throughout
- A. increase the substrate concentration
- B. supply the necessary coenzyme
- C. keep the pH constant throughout
- D. raise the temperature slowly
Explanation
Because pH changes can alter ionisation of the active site and denature the enzyme, a fair test holds pH steady with a buffer. Without one, the acid or alkali produced by the reaction itself would shift the result.
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About Characteristics of Enzymes
Enzymes are globular biological catalysts that lower activation energy without being consumed, usually showing specificity for particular substrates. Questions cover active sites, enzyme substrate complexes, the lock and key and induced fit models, intracellular and extracellular action, and the effects of denaturation, while distinguishing enzymes from non biological catalysts.
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