In an experiment on enzyme activity a buffer solution is used in order to
- A. increase the substrate concentration
- B. supply the necessary coenzyme
- C. keep the pH constant throughout
- D. raise the temperature slowly
Explanation
Because pH changes can alter ionisation of the active site and denature the enzyme, a fair test holds pH steady with a buffer. Without one, the acid or alkali produced by the reaction itself would shift the result.
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