Following is the gel pattern taken from Sangar's method of DNA sequencing. Read the sequence given in the gel
Correct answer: D. 3′-ATGCCAGTA-5′
- A. 5′-ATGCCAGTA-3′
- B. 5′-ATGACCGTA-3′
- C. 3′-TACGGTCAT-5′
- D. 3′-ATGCCAGTA-5′
Explanation
D is correct one. In the Sanger method, DNA sequencing is achieved by replicating the DNA strand in the presence of chain-terminating dideoxynucleotides (ddNTPs), which lack a 3'-OH group. This results in DNA fragments of varying lengths, terminated at specific nucleotides. These fragments are separated by size using gel electrophoresis, and the sequence is determined by reading the band pattern, with each band corresponding to a specific nucleotide position.
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About Structure of DNA
DNA structure includes nucleotides made of deoxyribose, phosphate and nitrogenous bases, arranged in two antiparallel polynucleotide strands. Questions cover the double helix, phosphodiester and hydrogen bonds, complementary base pairing between adenine and thymine and between guanine and cytosine, and the significance of major and minor grooves.
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