Experiment: In a classroom experiment, crushed liver (source of catalase) was placed in hydrogen peroxide solutions of different concentrations.Observation: Bubbles formed rapidly at 3% concentration, less at 1%, and very little at 0.2%.Question: What conclusion follows?
Correct answer: B. Catalase activity increases with substrate concentration up to a point.
- A. Hydrogen peroxide inhibits catalase at all levels.
- B. Catalase activity increases with substrate concentration up to a point.
- C. Liver tissue does not affect bubble formation.
- D. All concentrations give the same enzymatic activity.
Explanation
The correct answer is that catalase activity increases with substrate concentration up to a point. This conclusion is supported by the observation that more bubbles formed at higher hydrogen peroxide concentrations, indicating increased catalase activity. The other options are incorrect because they either misinterpret the relationship between catalase and hydrogen peroxide (inhibition or lack of effect) or suggest that all concentrations give the same enzymatic activity, which contradicts the experimental results.
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About Scientific Reasoning
Scientific reasoning evaluates hypotheses through observations, variables, controls, measurements and experimental results. Questions involve identifying independent and dependent variables, recognising a fair test, interpreting tables or graphs, distinguishing evidence from opinion, and deciding whether data supports, rejects or leaves a hypothesis unresolved.
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