Enzyme which attaches the Okazaki fragments in lagging strand is called:
Correct answer: D. DNA ligase
- A. Restriction endonuclease
- B. DNA helicase
- C. Primase
- D. DNA ligase
Explanation
Explanation:DNA Ligase is an enzyme that catalyzes the formation of a phosphodiester bond between adjacent Okazaki fragments on the lagging strand during DNA replication. This reaction joins the fragments together into a continuous strand. DNA Ligase is therefore essential for the completion of DNA replication on the lagging strand. Restriction endonuclease is an enzyme that cleaves DNA at specific sites called restriction sites. It is not involved in the attachment of Okazaki fragments during DNA replication. DNA helicase is an enzyme that unwinds the double-stranded DNA molecule at the replication fork during DNA replication. However, it is not involved in the attachment of Okazaki fragments during DNA replication.Primase is an enzyme that synthesizes a short RNA primer on the DNA template strand during DNA replication. However, it is not involved in the attachment of Okazaki fragments during DNA replication.
This question appeared on the UHS MDCAT 2009 paper, which you can sit online with every answer explained.
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DNA structure includes nucleotides made of deoxyribose, phosphate and nitrogenous bases, arranged in two antiparallel polynucleotide strands. Questions cover the double helix, phosphodiester and hydrogen bonds, complementary base pairing between adenine and thymine and between guanine and cytosine, and the significance of major and minor grooves.
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