Moderate

DNA polymerase adds nucleotides to the 3' end of the primer so the direction of replication will be?

Correct answer: A. 5' to 3'

  • A. 5' to 3'
  • B. 3' to 5'
  • C. 3' end of the primer to 3' end of template strand
  • D. 3' end of the template strand to the 3' end of the primer

Explanation

DNA polymerase is able to add nucleotides only in the 5′ to 3′ direction (a new DNA strand can be extended only in this direction). It also requires a free 3′-OH group to which it can add nucleotides by forming a phosphodiester bond between the 3′-OH end and the 5′ phosphate of the next nucleotide.

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