Which treatment permanently destroys the activity of an enzyme?
- A. Strong heating well above its optimum
- B. Cooling the solution to 0 degrees Celsius
- C. Diluting the enzyme solution
- D. Adding more substrate
Explanation
Strong heat breaks the weak bonds holding the tertiary structure, so the active site is permanently distorted. Cold only slows the molecules down and activity returns on warming, which is why refrigeration preserves without sterilising.
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