Which of the following technique is used to separate substances of high molecular weights (proteins, enzymes) of different charges?
Correct answer: B. Electrophoresis
- A. Dialysis
- B. Electrophoresis
- C. Solvent extraction
- D. Distillation
Explanation
Electrophoresis is a technique used to separate macromolecules like proteins based on their size and net electric charge. An electric field is applied to the sample, causing charged molecules to migrate at different rates.
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About Analytical Chemistry
Analytical chemistry identifies and measures substances using separation and instrumental methods. Chromatography is studied through stationary and mobile phases, retention and Rf values, while spectroscopy connects absorption of electromagnetic radiation with molecular structure and supports qualitative and quantitative analysis through techniques such as UV-visible and infrared spectroscopy.
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