Moderate

Which of the following is not step of recombinant DNA technology?

Correct answer: A. The cloning of gene of interest

  • A. The cloning of gene of interest
  • B. The genes of interest are cut down
  • C. The genes of interest are introduced into expression system
  • D. None of the above

Explanation

In order to produce recombinant DNA, the following are required: 1. Gene of interest, which is to be cloned. 2. Molecular scissors to cut out the gene of interest. 3. Molecular carrier or vector, on which gene of interest could be placed. 4. The gene of interest along with the vector is then introduced into an expression system, as a result of which a specific product is made.

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Biotechnology in health care applies biological organisms, cells or enzymes to diagnose and treat disease. The subject includes recombinant DNA, genetically engineered insulin, vaccines, antibiotics, monoclonal antibodies, gene therapy and techniques such as polymerase chain reaction, while distinguishing therapeutic applications from agricultural and industrial biotechnology.

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