Which method is used to sterilise heat-resistant surgical instruments by destroying microorganisms, including bacterial spores?

Correct answer: C. Processing in a steam autoclave

  • A. Wiping with a detergent solution
  • B. Soaking in a low-level disinfectant
  • C. Processing in a steam autoclave
  • D. Rinsing with sterile normal saline

Explanation

A steam autoclave uses pressurised moist heat to sterilise suitable heat-resistant equipment, including destruction of spores. Cleaning and disinfection reduce microorganisms but do not reliably achieve sterilisation.

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About Infection Prevention and Asepsis

Asepsis covers the chain of infection, hand hygiene, personal protective equipment, standard and transmission-based precautions, sterilisation, disinfection and safe waste disposal. The key distinction is between medical asepsis, which reduces microorganisms, and surgical asepsis, which maintains a sterile field during invasive procedures.

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