The viability of seeds is tested by
Correct answer: B. 2, 3, 5 triphenyl tetrazolium chloride
- A. 2, 6 dichlorophenol indophenols
- B. 2, 3, 5 triphenyl tetrazolium chloride
- C. DMSO
- D. Safranin
Explanation
Viability of seeds can be known by two methods: (i) ability to germinate, (ii) testing their ability to respire. All viable seeds respire. This can be tested by immersing a section of seed containing the embryo in 0.1% solution of triphenyl tetrazolium chloride. The viable embryo will turn pink due to conversion of colorless triphenyl tetrazolium chloride into insoluble colored dye called triphenyl formazone due to reduction.
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