The polyethylene glycol method is used for
Correct answer: D. Gene transfer without a vector.
- A. Biodiesel production
- B. Seedless fruit production
- C. Energy production from sewage
- D. Gene transfer without a vector.
Explanation
Direct gene transfer is the transfer of naked DNA into plant cells, but the presence of a rigid plant cell wall acts as a barrier to uptake. Therefore, protoplasts are the favoured target for direct gene transfer. Polyethylene glycol mediated DNA uptake is a direct gene transfer method that utilizes the interaction between PEG, naked DNA, salts and the protoplast membrane to effect transport of the DNA into the cytoplasm
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