The figure below shows three steps (A, B, C) of Polymerase Chain Reaction (PCR). Select the option giving correct identification together with what it represents?

Correct answer: C. C-extension in the presence of heat stable DNA polymerase

  • A. B-­denaturation at a temperature of about 98°C separating the two DNA strands
  • B. A-denaturation at a temperature of about 50°C
  • C. C-extension in the presence of heat stable DNA polymerase
  • D. A­-annealing with two sets of primers

Explanation

A schematic representation of PCR can be illustrated as follow.

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