Moderate

The commonly used fluorescent dye in the detection of tubercie bacilli________________?

Correct answer: D. Auramine and Rhodamine

  • A. Acridine orange
  • B. Thioglavin
  • C. Congo red
  • D. Auramine and Rhodamine

Explanation

Auramine-rhodamine fluorescent staining is used to screen sputum and other specimens for acid-fast Mycobacterium tuberculosis. The organisms fluoresce brightly against a darker background, improving detection compared with ordinary Ziehl-Neelsen staining.

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