Sterilisation control for moist heat is "or" The efficacy of the moist heat sterilization technique commonly used to sterlilize laboratory ware and culture media is tested by using_______________?

Correct answer: A. using Bacillus stearo thermo philus

  • A. using Bacillus stearo thermo philus
  • B. Using non-pathogenic strain of clostridium tetani
  • C. Using chemical indicator
  • D. Using methylene blue

Explanation

Bacillus stearothermophilus, now called Geobacillus stearothermophilus, is used as a biological indicator for moist-heat sterilisation because its spores are highly resistant to heat. Chemical indicators show exposure but do not reliably prove sterilising efficacy.

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