Some photosynthetic organisms contain chloroplasts that lack photosystem II yet can survive. The best way to detect the "lack of photosystem II" in these organisms would be:
Correct answer: B. To test for liberation of O₂ in the light
- A. To determine if they have thylakoids in the chloroplasts
- B. To test for liberation of O₂ in the light
- C. To test for CO2 fixation in the dark
- D. To do experiments to generate an action spectrum
Explanation
Photosystem II plays a vital role in the photolysis of water, which results in the release of oxygen. In organisms lacking Photosystem II, oxygen is not produced when exposed to light. Therefore, testing for oxygen liberation under light conditions is a direct and effective method to detect the absence of Photosystem II. Option A is incorrect because the presence of thylakoids does not confirm the presence of Photosystem II. Option C is incorrect, as CO₂ fixation in the dark is unrelated to Photosystem II. Option D is incorrect because an action spectrum does not specifically address the presence of Photosystem II.
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