Pasteur applied the principle of inoculation with attenuated cultures for the prevention of:

Explanation

Pasteur made many discoveries concerning the cause and prevention of infectious diseases. In 1880s he isolated the bacterium responsible for chicken cholera. He grew it in a pure culture. To prove that he really had isolated the bacterium responsible for this disease Pasteur made use of the fundamental techniques devised by Koch. He arranged experiments for public demonstration in which he repeated an experiment that had been successful in many previous trails in his laboratory. Unluckily his demonstration failed badly, as he had used an attenuated culture for that. It was accidentally proved that attenuated culture of some pathogenic bacteria will be unable to cause infection, however it will be capable enough to stimulate the immune system to synthesize antibodies. Pasteur next applied this principle of inoculation with attenuated cultures to the prevention of Anthrax.