Moderate

Km of an enzyme is_____________?

Correct answer: C. Substrate concentration at half maximal velocity

  • A. Dissociation constant
  • B. The normal physiological substrate concentration
  • C. Substrate concentration at half maximal velocity
  • D. Numerically identical for all isozymes that catalyses a given reaction

Explanation

Km is the substrate concentration at which an enzyme reaches half of its maximum velocity, Vmax. It reflects apparent substrate affinity, but it is not always identical to the true dissociation constant.

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