In glycolysis, during oxidation electrons are removed by:

Explanation

In glycolysis, NAD+ plays a crucial role as an electron carrier. During the oxidation of glyceraldehyde-3-phosphate to 1,3-bisphosphoglycerate, electrons are transferred to NAD+, reducing it to NADH. This step is essential for continuing glycolysis as it helps in the extraction of energy from glucose. Molecular oxygen is not used in glycolysis; it is the terminal electron acceptor in the electron transport chain. ATP is generated rather than used for electron removal in glycolysis. Glyceraldehyde-3-phosphate, while undergoing oxidation, transfers electrons but is not the acceptor itself.