If the primer annealing temperature is increased to 94°C . What will happen ?
Correct answer: C. No Annealing
- A. Annealing
- B. Extension
- C. No Annealing
- D. Primer-dimer formation
Explanation
Primer annealing is a critical step in polymerase chain reaction or PCR. In this step, the primers bind to flanking sequences of the target DNA for amplification. If the primer annealing temperature is increased to 94°C non annealing occurs. At high temperature Non annealing occurs and the whole process is disturbed.
Report an error
The more specific you are, the faster it gets fixed. A source beats an opinion.
Prefer email? [email protected]
About Biological Molecules
The chapter covers water's polarity and biological roles, carbohydrates, lipids, proteins and nucleic acids, including their monomers, bonds, structures and functions. It also distinguishes RNA from DNA and explains conjugated molecules such as glycoproteins, glycolipids and nucleoproteins, which combine organic molecules with a non-protein component.
Practise Biological Molecules
1,790 free Biological Molecules MCQs from Biology, each with the correct answer and an explanation. Unlimited attempts, no account needed.
Discussion
Stuck on an option, or know a faster way to get there? Ask or explain it here.
No comments yet. Be the first to explain this one.
Exams that ask Biology questions like this
Biology is on 8 papers prepared for on TestUstad, and all of them draw the same bank, so this question is worth knowing for every one of them.
More Biological Molecules questions
Which one of the following is addition polymer:
During the formation of addition polymers, which smaller molecules you think are eliminated:
Amino acid leucine is coded by how many codons:
Which one of the following is called animal starch?
Enzymes are _that catalyze chemical reactions in living organisms and are very specific in their action:
In RNA, which of the bases is replaced by uracil?