Given are the steps of DNA fingerprinting (southern blot test).I. X-ray film.II. Electrophoresis.III. Radioactive probing.IV. Digestion with restriction enzyme.The CORRECT sequence in which these steps are followed is:
Correct answer: C. IV→ II→ III→ I
- A. IV→III→ II→ I
- B. III→ IV→ I→ II
- C. IV→ II→ III→ I
- D. I→ IV→ III→ II
Explanation
The correct sequence for DNA fingerprinting using the Southern blot test is IV→ II→ III→ I. This process begins with digestion with restriction enzyme (IV), which cuts the DNA into fragments. Next, these fragments are separated by size using electrophoresis (II). The separated DNA fragments are then transferred to a membrane and hybridized with a radioactive probe (III) that binds to specific sequences. Finally, the pattern of the labeled fragments is visualized using X-ray film (I). Other options are incorrect as they do not follow this established sequence.
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