__________ can denature enzymes resulting in slow catalysis.

Correct answer: B. High temperature

  • A. Low temperature
  • B. High temperature
  • C. High water content
  • D. Low water content

Explanation

High temperature breaks the hydrogen and ionic bonds holding an enzyme's shape, so the active site is lost and the enzyme is denatured. Low temperature only slows the enzyme down reversibly without changing its structure, and water content does not denature it.

This question appeared on the UHS MDCAT 2026 paper, which you can sit online with every answer explained.

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About Factors Affecting Enzyme Action

Enzyme activity changes with temperature, pH, substrate concentration and enzyme concentration because these factors affect molecular collisions and the active site. Questions also examine optimum conditions, denaturation and inhibition, while distinguishing a fall in activity caused by unsuitable conditions from permanent enzyme damage.

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